Application
IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1 antibody diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | IGF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IGF1 (insulin-like growth factor 1 (somatomedin C))The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, Bovine, Guinea pigMouse (93%), Rat (94%), Xenopus laevis (80%), Dog (98%), Pig (96%), Rabbit (82%), Chicken (85%), Sheep (96%), Rhesus Monkey (100%), Bovine (97%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IGF-I antibody, IGF1A antibody, IGFI antibody, IGF1 antibody, mechano growth factor antibody, IGF-IA antibody, somatomedin-C antibody, insulin-like growth factor I antibody, insulin-like growth factor IA antibody, MGF antibody, insulin-like growth factor I |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1 antibody diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
