Application
IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: IGF2 protein stained by IGF2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
HepG2 whole cell extract and conditioned medium (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] diluted at 1:500.Application
IGF2 antibody [N1C3] detects IGF2 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. IGF2 antibody [N1C3] dilution: 1:500.| Product Name | IGF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IGF2 (insulin-like growth factor 2 (somatomedin A))This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IGF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Pig, Sheep, BovineHuman (100%), Rat (85%), Dog (96%), Pig (92%), Sheep (87%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C11orf43 antibody, FLJ22066 antibody, FLJ44734 antibody, IGF-II antibody, PP9974 antibody, IGF2 antibody, insulin-like growth factor type 2 antibody, somatomedin-A antibody, insulin-like growth factor II antibody, insulin-like growth factor 2 (somatomedin |
|---|---|
| Target | IGF2 |
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Secreted |
Application
IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: IGF2 protein stained by IGF2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
HepG2 whole cell extract and conditioned medium (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] diluted at 1:500.
Application
IGF2 antibody [N1C3] detects IGF2 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. IGF2 antibody [N1C3] dilution: 1:500.| Application Notes | Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
