Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IGF2BP2 antibody at 1:250 dilution.Application
IGF2BP2 antibody detects IGF2BP2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE IGF2BP2 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IGF2BP2 antibody [N1N3] detects IGF2BP2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | IGF2BP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IGF2BP2 (insulin-like growth factor 2 mRNA binding protein 2)This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IGF2BP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (92%), Rat (92%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IMP-2 antibody, IMP2 antibody, VICKZ2 antibody, p62 antibody, IGF2BP2 antibody, VICKZ family member 2 antibody, IGF2 mRNA-binding protein 2 antibody, hepatocellular carcinoma autoantigen p62 antibody, IGF-II mRNA-binding protein 2 antibody, insulin-like gr |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) (3) |
| Cellular Localization | Cytoplasm (Probable) |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IGF2BP2 antibody at 1:250 dilution.
Application
IGF2BP2 antibody detects IGF2BP2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE IGF2BP2 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IGF2BP2 antibody [N1N3] detects IGF2BP2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33343 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
