Application
Flow cytometric Analysis of Hela cells, using anti-IGF2BP2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Flow cytometric Analysis of Jurkat cells, using anti-IGF2BP2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC205673 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.Application
Anti-IGF2BP2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IGF2BP2Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.| Product Name | IGF2BP2 antibody [3G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3G7] to IGF2BP2Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. |
| Immunogen | Full length human recombinant protein of human IGF2BP2(NP_006539) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G7 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | VICKZ2, IGF2BP2, p62, IMP2 |
|---|---|
| Target | IGF2BP2 |
| Tissue Specificity | is specific for human IGF2BP2(NP_006539). |
| Cellular Localization | Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. |
Application
Flow cytometric Analysis of Hela cells, using anti-IGF2BP2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Flow cytometric Analysis of Jurkat cells, using anti-IGF2BP2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC205673 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.
Application
Anti-IGF2BP2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IGF2BP2
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
