Application
Immunoprecipitation of IGFBP1 protein from HepG2 whole cell extracts using 5 μg of IGFBP1 antibody . Western blot analysis was performed using IGFBP1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP1 antibody diluted at 1:1000.| Product Name | IGFBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IGFBP1 (insulin-like growth factor binding protein 1)This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IGFBP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PP12 , hIGFBP-1 , IGF-BP25 , insulin-like growth factor binding protein 1 , IBP1 , AFBP |
|---|---|
| Target | IGFBP1 |
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Secreted |
Application
Immunoprecipitation of IGFBP1 protein from HepG2 whole cell extracts using 5 μg of IGFBP1 antibody . Western blot analysis was performed using IGFBP1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP1 antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
