Application
WB analysis of Ramos and Jurkat lysates using Ikaros antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysate using control IgG and Ikaros antibody. The precipitates were detected by the same Ikaros antibody.| Product Name | Ikaros antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1A12-F2-D8] to IkarosTranscription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs). |
| Immunogen | Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A12-F2-D8 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Hs.54452 PRO0758 hIk-1 IK1 IKAROS family zinc finger 1 (Ikaros) IKAROS IKZF1 LYF1 ZNFN1A1 |
|---|---|
| Target | Ikaros |
| Molecular Weight(MW) | 58kDa (note) |
| Cellular Localization | Nucleus |
Application
WB analysis of Ramos and Jurkat lysates using Ikaros antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysate using control IgG and Ikaros antibody. The precipitates were detected by the same Ikaros antibody.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
