Application
Western blot analysis of IKB alpha (pS32/S36) expression in HepG2 TNFa-treated (A); Raw264.7 TNFa-treated (B); PC12 TNFa-treated (C) whole cell lysates.Application
Immunohistochemical analysis of IKB alpha (pS32/S36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Application
Immunofluorescent analysis of IKB alpha (pS32/S36) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Product Name | IKB alpha (phospho Ser32/Ser36) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKB alpha (phospho Ser32/Ser36)This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKB alpha. The exact sequence is proprietary. |
| Modification | p-Ser32/Ser36 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IKBA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha MAD-3 NFKBIA NFKBI |
|---|---|
| Target | IKB alpha (phospho Ser32/Ser36) |
| Tissue Specificity | Recognizes endogenous levels of IKB alpha (pS32/S36) protein. |
Application
Western blot analysis of IKB alpha (pS32/S36) expression in HepG2 TNFa-treated (A); Raw264.7 TNFa-treated (B); PC12 TNFa-treated (C) whole cell lysates.
Application
Immunohistochemical analysis of IKB alpha (pS32/S36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Application
Immunofluorescent analysis of IKB alpha (pS32/S36) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
