ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-IKB alpha (Tyr42) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Tyr42 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIA |
|---|---|
| Gene Synonyms | IKBA MAD-3 NFKBI EDAID2 |
| Gene Full Name | NFKB inhibitor alpha |
| Gene Summary | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Alternative Names | I kappa B alpha I-kappa-B-alpha IkappaBalpha IkB-alpha IKBA IKBA_HUMAN IKBalpha MAD 3 MAD3 Major histocompatibility complex enhancer-binding protein MAD3 NF kappa B inhibitor alpha NF-kappa-B inhibitor alpha NFKBI NFKBIA Nuclear factor of kappa light chain gene enhancer in B cells Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha |
| Molecular Weight(MW) | 36kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P25963 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
