Application
Western blot analysis of extracts from COS-7 cells using I kappaB- alpha (Phospho-Tyr305) Antibody. The lane on the left is treated with the antigen-specific peptide.| Product Name | IKB alpha (phospho Tyr305) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKB alpha (phospho Tyr305)This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 305(L-P-Y(p)-D-D) derived from Human I kappaB- alpha. |
| Modification | p-Tyr305 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAD-3 IKBA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha NFKBIA NFKBI |
|---|---|
| Target | IKB alpha (phospho Tyr305) |
| Tissue Specificity | The antibody detects endogenous level of I kappaB- alpha only when phosphorylated at Tyrosine 305. |
Application
Western blot analysis of extracts from COS-7 cells using I kappaB- alpha (Phospho-Tyr305) Antibody. The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
