Application
Western blot analysis of extracts of various cell lines, using NFKBIA antibody.Application
Immunohistochemistry of paraffin-embedded mouse kidney using NFKBIA antibody at dilution of 1:100 (400x lens).Application
Immunofluorescence analysis of HeLa cell using NFKBIA antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of U2OS cell using NFKBIA antibody. Blue: DAPI for nuclear staining.| Product Name | IKB alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKB alphaThis gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | Recombinant protein of human NFKBIA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:100, IP 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAD-3 NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha IKBA NFKBI |
|---|---|
| Target | IKB alpha |
| Molecular Weight(MW) | 36 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using NFKBIA antibody.
Application
Immunohistochemistry of paraffin-embedded mouse kidney using NFKBIA antibody at dilution of 1:100 (400x lens).
Application
Immunofluorescence analysis of HeLa cell using NFKBIA antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of U2OS cell using NFKBIA antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:100, IP 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
