Application
Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IKB alpha antibody detects IKB alpha protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extrac 10% SDS-PAGE IKB alpha antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha antibody at 1:200 dilution.Application
IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IKB alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKB alpha (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha)NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, BovineRat (91%), Pig (95%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKBA antibody MAD-3 antibody NFKBI antibody NFKBIA antibody NF-kappa-B inhibitor alpha antibody IkappaBalpha antibody I-kappa-B-alpha antibody nuclear factor of kappa light chain gene enhancer in B-cells antibody ikB-alpha antibody major histocomp |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IKB alpha antibody detects IKB alpha protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extrac 10% SDS-PAGE IKB alpha antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha antibody at 1:200 dilution.
Application
IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , BCL-1 , C2C12 , HT-29 , NIH-3T3 , *MCF7 , *RAW264.7 , *B16F10 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
