Application
Western blot analysis of IKK beta (pT19) expression in Raw264.7 LPS-treated (A); PC12 UV-treated (B); rat brain (C) whole cell lysates.Application
Immunohistochemical analysis of IKK beta (pT19) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | IKB beta (phospho Thr19) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKB beta (phospho Thr19)The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKK beta. The exact sequence is proprietary. |
| Modification | p-Thr19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IKBB nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor beta TRIP9 NFKBIB |
|---|---|
| Target | IKB beta (phospho Thr19) |
| Tissue Specificity | Recognizes endogenous levels of IKK beta (pT19) protein. |
Application
Western blot analysis of IKK beta (pT19) expression in Raw264.7 LPS-treated (A); PC12 UV-treated (B); rat brain (C) whole cell lysates.
Application
Immunohistochemical analysis of IKK beta (pT19) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
