Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB- epsilon (Phospho-Ser22) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB- epsilon (Phospho-Ser22) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody| Product Name | IkB epsilon (phospho Ser22) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IkB epsilon (Phospho Ser22)Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. |
| Immunogen | Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB- epsilon . |
| Modification | p-Ser22 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKBE, NFKBIE, ikappaBepsilon, NF-kappa-B inhibitor epsilon, ikB-epsilon, I-kappa-B-epsilon, ikB-E, NF-kappa-BIE, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon |
|---|---|
| Target | IkB epsilon (Phospho Ser22) |
| Molecular Weight(MW) | 40 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB- epsilon (Phospho-Ser22) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB- epsilon (Phospho-Ser22) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
