Application
IKAP antibody [C1C2], Internal detects IKAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IKAP antibody [C1C2], Internal detects IKAP protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract 5% SDS-PAGE IKAP antibody [C1C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IKBKAP antibody [C1C2], Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKBKAP (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein)The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IKBKAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, RabbitRat (85%), Rabbit (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp781H1425 antibody, DYS antibody, ELP1 antibody, FD antibody, FLJ12497 antibody, IKAP antibody, IKI3 antibody, TOT1 antibody, IKBKAP antibody, p150 antibody, ikappaB kinase complex-associated protein antibody, IKK complex-associated protein antibody, e |
|---|---|
| Molecular Weight(MW) | 150 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
IKAP antibody [C1C2], Internal detects IKAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IKAP antibody [C1C2], Internal detects IKAP protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract 5% SDS-PAGE IKAP antibody [C1C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
