Application
Immunofluorescence analysis of methanol-fixed HeLa, using IKBKE antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000Application
IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm in human endometrium by immunohistochemical analysis. Sample: Paraffin-embedded human endometrium. IKBKE antibody [C2C3], C-term diluted at 1:500.Application
IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IKBKE protein stained by IKBKE antibody [C2C3], C-term diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | IKBKE antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKBKE (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon)IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human IKBKE. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKK-E antibody IKK-i antibody IKKE antibody IKKI antibody KIAA0151 antibody MGC125294 antibody MGC125295 antibody MGC125297 antibody IKBKE antibody inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody IKK-related kinase epsilon ant |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using IKBKE antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000
Application
IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm in human endometrium by immunohistochemical analysis. Sample: Paraffin-embedded human endometrium. IKBKE antibody [C2C3], C-term diluted at 1:500.
Application
IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IKBKE protein stained by IKBKE antibody [C2C3], C-term diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
