Application
Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents the isotype control antibody, red represents the IKK alpha antibody. Intracellular flow kit was used for this test, and an anti-mouse IgG1: PE conjugated secondary antibody.Application
Formalin-fixed, paraffin-embedded human kidney stained with IKK alpha antibody at 5 ug/ml.Application
Western blot analysis of 30 g of total cell lysate from Daudi cells with at 1 ug/ml dilution.| Product Name | IKK alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [14A231] to IKK alphaNFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFk B is negatively modulated by the inhibitory proteins Ik-B. In turn Ik-B is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK alpha and IKK beta which phosphorylate Ik-B leading to its degradation and the resulting translocation of NFk B to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF alpha , endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 14A231 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FIHC-PIPWB |
|
IHC: 5ug/ml : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKBKA IKK-alpha IKK1 IKKA NFKBIKA TCF16 IKK alpha IKKalpha |
|---|---|
| Target | IKK alpha |
| Cellular Localization | Cytoplasmic |
Application
Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents the isotype control antibody, red represents the IKK alpha antibody. Intracellular flow kit was used for this test, and an anti-mouse IgG1: PE conjugated secondary antibody.
Application
Formalin-fixed, paraffin-embedded human kidney stained with IKK alpha antibody at 5 ug/ml.
Application
Western blot analysis of 30 g of total cell lysate from Daudi cells with at 1 ug/ml dilution.| Positive Control | Daudi Whole cell extract |
|---|---|
| Application Notes |
IHC: 5ug/ml : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.05% Sodium Azide |
