| Product Name | IKK beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IKK betaNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK), Which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for NF-kB activation. IKKb phosphorylates both IkB-a and IkB-b. IKKb is expressed in variety of human tissues. |
| Immunogen | Synthetic peptide - amino acids 662-680 of human IKK beta which differs from the corresponding murine sequence by one amino acid |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ33771 FLJ36218 FLJ38368 FLJ40509 IKK-beta IKK2 IKKB MGC131801 NFKBIKB IKK beta IKKbeta |
|---|---|
| Target | IKK beta |
| Tissue Specificity | Recognises IKK beta and does not cross react with IKK alpha or IKK gamma |
| Cellular Localization | Cytoplasmic |
| Positive Control | Jurkat whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
