Application
Western blot analysis of extracts from MDA cells using IKK- beta Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using IKK- beta Antibody .| Product Name | IKK beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK betaActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. |
| Immunogen | Peptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK- beta . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ33771 FLJ36218 FLJ38368 FLJ40509 IKK-beta IKK2 IKKB MGC131801 NFKBIKB IKBKB IKK-B I-kappa-B kinase 2 I-kappa-B-kinase beta nuclear factor NF-kappa-B inhibitor kinase beta inhibitor of nuclear factor kappa-B kinase subunit beta inhibitor o |
|---|---|
| Target | IKK beta |
| Molecular Weight(MW) | 87 (note) |
Application
Western blot analysis of extracts from MDA cells using IKK- beta Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using IKK- beta Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
