Application
Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-IKK-gamma (Ser85) antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | IKK gamma (phospho Ser85) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK gamma (phospho Ser85)This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Aug 2011] |
| Immunogen | Peptide sequence around phosphorylation site of serine 85 (Q-A-S(p)-Q-R) derived from Human IKK-gamma. |
| Modification | p-Ser85 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKK-gamma ZC2HC9 FIP3 FIP-3 AMCBX1 NEMO IKBKG Fip3p IP1 inhibitor of kappa light polypeptide gene enhancer in B-cells kinase gamma IP2 |
|---|---|
| Target | IKK gamma (phospho Ser85) |
| Tissue Specificity | The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85. |
Application
Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-IKK-gamma (Ser85) antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
