Application
Western blot analysis of IKK gamma (pS85) expression in THP1 LPS-treated (A); Raw264.7 LPS-treated (B); rat brain (C) whole cell lysates.Application
Immunohistochemical analysis of IKK gamma (pS85) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | IKK gamma (phospho Ser85) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK gamma (phospho Ser85)This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Aug 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKK gamma. The exact sequence is proprietary. |
| Modification | p-Ser85 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ZC2HC9 IKBKG inhibitor of kappa light polypeptide gene enhancer in B-cells kinase gamma AMCBX1 IP FIP-3 Fip3p IKK-gamma IP1 FIP3 NEMO |
|---|---|
| Target | IKK gamma (phospho Ser85) |
| Tissue Specificity | Recognizes endogenous levels of IKK gamma (pS85) protein. |
Application
Western blot analysis of IKK gamma (pS85) expression in THP1 LPS-treated (A); Raw264.7 LPS-treated (B); rat brain (C) whole cell lysates.
Application
Immunohistochemical analysis of IKK gamma (pS85) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
