Application
Western blot analysis of IKK-g in Interlukin-1-stimulated 293 cells.| Product Name | IKK gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to IKK - gammaPro-inflammatory cytokines activate the transcription factor NF-kappaB by stimulating the activity of a protein kinase that phosphorylates IkappaB, an inhibitor of NF-kappaB, at sites that trigger its ubiquitination and degradation. A large, cytokine-responsive IkappaB kinase (IKK) complex contains 2 subunits, IKK-alpha and IKK-beta, which are protein kinases whose function is needed for NF-kappaB activation by pro-inflammatory stimuli. IKK is composed of similar amounts of IKK-alpha, IKK-beta, which are differentially processed forms of a third subunit, IKK-gamma. IKK-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | IKK gamma |
|---|---|
| Tissue Specificity | Reacts with the residues 2-13 [NRHLWKSQLCEM] of the IKK-g protein. Recognizes both human isoforms. |
Application
Western blot analysis of IKK-g in Interlukin-1-stimulated 293 cells.| Application Notes | Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
