Application
IKK gamma antibody detects IKBKG protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IKBKG protein stained by IKK gamma antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using IKK gamma antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLaS3 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE diluted at 1:1000| Product Name | IKK gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK gamma (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma)This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [supplied by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IKK gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rabbit, BovineRat (90%), Pig (87%), Rabbit (92%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMCBX1 antibody FIP-3 antibody FIP3 antibody Fip3p antibody IKK-gamma antibody IP antibody IP1 antibody IP2 antibody IPD2 antibody NEMO antibody IKBKG antibody inhibitor of nuclear factor kappa-B kinase subunit gamma antibody incontinentia pigm |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
IKK gamma antibody detects IKBKG protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IKBKG protein stained by IKK gamma antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using IKK gamma antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLaS3 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE diluted at 1:1000| Positive Control | HeLaS3 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
