Application
Western blot analysis of IKK-g in Interleukin-1 stimulated 293 cells with anti-IKK-g at a 1:500 dilution.Application
Detection of IKK-g using as the capture antibody in ELISA| Product Name | IKK gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IKK ( gamma ) C - termPro-inflammatory cytokines activate the transcription factor NF-kappa B by stimulating the activity of a protein kinase that phosphorylates Ikappa B, an inhibitor of NF-kappa B, at sites that trigger its ubiquitination and degradation. A large, cytokine-responsive Ikappa B kinase (IKK) complex has been purified and the genes encoding 2 of its subunits have been cloned. These subunits, IKK-alpha and Ikk-beta, are protein kinases whose function is needed for NF-kappa B activation by pro-inflammatory stimuli. IKK is composed of similar amounts of IKK-alpha, Ikk-beta, which are differentially processed forms of a third subunit, IKK-gamma. Ikk-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex. |
| Immunogen | C-terminus of the IKK-gamma protein (proprietary) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | IKK gamma |
|---|---|
| Tissue Specificity | Reacts with residues 385-399 [RRSPPEEPPDFCCPK] of the IKK-g protein. Recognizes both human isoforms. |
Application
Western blot analysis of IKK-g in Interleukin-1 stimulated 293 cells with anti-IKK-g at a 1:500 dilution.
Application
Detection of IKK-g using as the capture antibody in ELISA| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
