Application
Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract, CM: conditioned medium)| Product Name | IL1 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL1 beta (interleukin 1, beta)The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rabbit, Rhesus MonkeyRabbit (80%), Rhesus Monkey (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IL1F2 IL-1 interleukin 1 beta IL1B IL1-BETA |
|---|---|
| Target | IL1 beta |
| Molecular Weight(MW) | 31 kDa (note) |
| Cellular Localization | Secreted |
Application
Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract, CM: conditioned medium)| Positive Control | THP-1 (500ng/ml LPS 4hr in serum free, 1mM ATP 1.5hr) , THP-1 ( 500ng/ml LPS 4hr in serum free，1mM ATP 1.5hr Culture medium) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
