Application
IHC-P analysis of human lung carcinoma using IL1 beta antibody.Application
Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of IL1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab.| Product Name | IL1 beta antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IL1 betaThe protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 154~183 amino acids from the Center region of human IL1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IL1F2 Catabolin Interleukin-1 beta IL1B IL1 beta Interleukin1 beta IL-1 beta IL 1 beta Interleukin 1 beta |
|---|---|
| Target | IL1 beta |
| Molecular Weight(MW) | 30.748 kDa (note) |
| Cellular Localization | The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
Application
IHC-P analysis of human lung carcinoma using IL1 beta antibody.
Application
Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of IL1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
