Application
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist antibody at 1:500 dilution.Application
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis. A. 30 µg Raji whole cell lysate/extract 15% SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 15% SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 15% SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist antibody at 1:200 dilution.| Product Name | IL1 Receptor antagonist antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL1 Receptor antagonist (interleukin 1 receptor antagonist)The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, BovineDog (83%), Pig (82%), Bovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DIRA antibody ICIL-1RA antibody IL-1RN antibody IL-1ra antibody IL-1ra3 antibody IL1F3 antibody IL1RA antibody IRAP antibody MGC10430 antibody MVCD4 antibody IL1RN antibody IL1 inhibitor antibody IL1RN (IL1F3) antibody interleukin-1 receptor a |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) (5) |
| Cellular Localization | Isoform 1: Secreted , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 4: Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist antibody at 1:500 dilution.
Application
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis. A. 30 µg Raji whole cell lysate/extract 15% SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 15% SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 15% SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist antibody at 1:200 dilution.| Positive Control | Raji , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
