Application
Sample(30 µg whole cell lysate) A:A431 B: HepG2 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IL12 Receptor beta2 antibody , Internal |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IL-12 Receptor beta2 (interleukin 12 receptor, beta 2)The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL-12 Receptor beta2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Dog, Pig, BovineDog (80%), Pig (80%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IL12RB2 antibody interleukin-12 receptor beta-2 chain antibody IL-12 receptor subunit beta-2 antibody interleukin-12 receptor subunit beta-2 antibody IL-12R subunit beta-2 antibody IL-12RB2 antibody IL-12 receptor beta 2 antibody IL-12R-beta-2 antib |
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| Target | IL-12 Receptor beta2 |
| Molecular Weight(MW) | 97 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample(30 µg whole cell lysate) A:A431 B: HepG2 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
