| Product Name | IL13 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [32116.11] to IL13Human IL13 was originally identified by differential screening of an anti-CD28 activated human peripheral blood mononuclear cell cDNA library as an induction-specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1-like, Th2-like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. |
| Immunogen | Recombinant full length human IL13 protein expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 32116.11 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at a concentration of 4 μg/ml (100 ul/well) in combination with a biotinylated IL13 polyclonal detection antibody at 400 ng/ml (100 μl/well), an ELISA for sample volumes of 100 μl with a range of 62.5-4000 ug/ml can be obtained. Neut: Use at a concentration of 1-3 μg/ml in the presence of 10 ng/ml of recombinant human IL13 using the human erythroleukemia cell line, TF-1. WB: Use at a concentration of 1-2 μg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 12 kDa). The detection limit for recombinant human IL13 is approximately 50 ng/lane under non-reducing and reducing conditions. Endotoxin level is: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | ALRH BHR1 IL-13 MGC116786 MGC116788 MGC116789 P600 IL 13 IL13 |
|---|---|
| Target | IL13 |
| Cellular Localization | Secreted |
| Positive Control | GTX10782 has the ability to neutralize the bioactivity of human IL13 in the human erythroleukemia cell line, TF-1 |
|---|---|
| Application Notes | ELISA: Use at a concentration of 4 μg/ml (100 ul/well) in combination with a biotinylated IL13 polyclonal detection antibody at 400 ng/ml (100 μl/well), an ELISA for sample volumes of 100 μl with a range of 62.5-4000 ug/ml can be obtained. Neut: Use at a concentration of 1-3 μg/ml in the presence of 10 ng/ml of recombinant human IL13 using the human erythroleukemia cell line, TF-1. WB: Use at a concentration of 1-2 μg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 12 kDa). The detection limit for recombinant human IL13 is approximately 50 ng/lane under non-reducing and reducing conditions. Endotoxin level is: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Constituents: PBS |
