| Product Name | IL18 Receptor 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [70625.111] to IL18R1IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. |
| Immunogen | Recombinant full length human IL18R1 protein expressed in mouse NSO cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 70625.111 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| I-ELISA: Use at a concentration of 0.5-1 μg/ml, determined to detect a limit of ~6 ng/well of recombinant human IL18R1. Neut: Use at a concentration of 0.12 μg/ml. OM258881 will neutralize 50% of the bioactivity due to 50 ng/ml recombinant human IL18 using activated human T cells. WB: Use at a concentration of 1-2 μg/ml, determined using human IL18R1 at 25 ng/lane under non-reducing conditions. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | IL18 R1 IL18R1 CD218a CDw218a IL-1Rrp IL18RA IL1RRP IL 1Rrp |
|---|---|
| Target | IL18-R1 |
| Cellular Localization | Plasma membrane |
| Positive Control | To measure the ability of GTX10788 to neutralize the bioactivity of human IL18R1, various concentrations of the antibody were incubated with PHA and IL12-activated T cells |
|---|---|
| Application Notes | I-ELISA: Use at a concentration of 0.5-1 μg/ml, determined to detect a limit of ~6 ng/well of recombinant human IL18R1. Neut: Use at a concentration of 0.12 μg/ml. OM258881 will neutralize 50% of the bioactivity due to 50 ng/ml recombinant human IL18 using activated human T cells. WB: Use at a concentration of 1-2 μg/ml, determined using human IL18R1 at 25 ng/lane under non-reducing conditions. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Constituents: PBS |
