Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL1RAP antibody at 1:500 dilution.Application
HepG2 whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IL1RAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL1RAP (interleukin 1 receptor accessory protein)Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes an interleukin 1 receptor accessory protein. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL1RAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanPig |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (87%), Rat (87%), Rhesus Monkey (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C3orf13 antibody FLJ37788 antibody IL-1RAcP antibody IL1R3 antibody IL1RAP antibody IL-1R3 antibody interleukin-1 receptor accessory protein antibody interleukin-1 receptor accessory protein beta antibody IL-1 receptor accessory protein antibody i |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (6) |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted , Isoform 3: Secreted |
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL1RAP antibody at 1:500 dilution.
Application
HepG2 whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
