Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using IL2 Receptor beta antibody at 1:100 dilution.Application
Sample(30 μg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1000Application
IL2 Receptor beta antibody [C3], C-term detects IL2RB protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL2RB protein stained by IL2 Receptor beta antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | IL2 Receptor beta antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL2 Receptor beta (interleukin 2 receptor, beta)The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL2 Receptor beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD122 antibody IL15RB antibody P70-75 antibody IL2RB antibody IL-2 receptor subunit beta antibody IL-2RB antibody interleukin-2 receptor subunit beta antibody high affinity IL-2 receptor subunit beta antibody high affinity IL-2 receptor beta subuni |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using IL2 Receptor beta antibody at 1:100 dilution.
Application
Sample(30 μg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1000
Application
IL2 Receptor beta antibody [C3], C-term detects IL2RB protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL2RB protein stained by IL2 Receptor beta antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
