Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using IL27 antibody at 1:500 dilution.Application
IL27 antibody detects IL27 protein by western blot analysis. Non-transfected (-) and IL27 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL27 antibody diluted by 1:1000.| Product Name | IL27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL27 (interleukin 27)The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL27. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IL-27 antibody IL-27A antibody IL27p28 antibody IL30 antibody MGC71873 antibody p28 antibody IL27 antibody IL-27 p28 subunit antibody IL27-A antibody interleukin-27 subunit alpha antibody IL-27-A antibody IL-27 subunit alpha antibody interleuki |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using IL27 antibody at 1:500 dilution.
Application
IL27 antibody detects IL27 protein by western blot analysis. Non-transfected (-) and IL27 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL27 antibody diluted by 1:1000.| Positive Control | 3XFlag-human IL-27 -transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
