Application
Western blot analysis of 35ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-IL-3 antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3.| Product Name | IL3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5G7H9] to IL-3Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 20-152 of human IL-3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5G7H9 |
| Host Species | Mouse |
| Tested Applications | WB |
| IHC 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MCGF IL3 MULTICSF MGC79399 MULTI-CSF IL-3 MGC79398 IL 3 MULTI CSF |
|---|---|
| Target | IL-3 |
| Tissue Specificity | is specific for amino acids 20-152 of human IL-3 |
Application
Western blot analysis of 35ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-IL-3 antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3.| Application Notes | IHC 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
