Application
IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein by western blot analysis. THP-1 whole cell extracts (30 μg), non-transfected (-) and IL3 Receptor alpha-transfected (+) 293T whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL3 Receptor alpha antibody diluted by 1:1000.Application
IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein at cell membrane and cytoplasm in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. IL3 Receptor alpha antibody [N2C2], Internal diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL3 Receptor alpha antibody at 1:250 dilution.| Product Name | IL3 Receptor alpha antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL3 Receptor alpha (interleukin 3 receptor, alpha (low affinity))The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL3 Receptor alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD123 antibody IL3R antibody IL3RAY antibody IL3RX antibody IL3RY antibody MGC34174 antibody hIL-3Ra antibody IL3RA antibody CD123 antigen antibody IL-3R-alpha antibody IL-3RA antibody interleukin-3 receptor subunit alpha antibody IL-3 receptor |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein by western blot analysis. THP-1 whole cell extracts (30 μg), non-transfected (-) and IL3 Receptor alpha-transfected (+) 293T whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL3 Receptor alpha antibody diluted by 1:1000.
Application
IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein at cell membrane and cytoplasm in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. IL3 Receptor alpha antibody [N2C2], Internal diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL3 Receptor alpha antibody at 1:250 dilution.| Positive Control | THP-1 , IL3 Receptor alpha-transfected (+) 293T whole cell extracts |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
