| Product Name | IL4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3H4] to IL4The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H4 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-P |
| ELISA: Use at a concentration of 2 µg/ml as the capture reagent in a two-site sandwich ELISA for measuring human IL4 levels. The detection limit is 5 pg/ml ranging up to 3 ng/ml. ICC: Use at an assay dependent dilution. GTX13778 recognizes the secreted (membrane-bound) form of IL4. GTX13778 can be used for intracellular staining of gradient purified human basophils stimulated with IL3. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | BCGF-1 BCGF1 BSF1 IL-4 MGC79402 IL 4 BCGF 1 IL4 |
|---|---|
| Target | IL4 |
| Cellular Localization | Secreted |
| Positive Control | Recombinant human IL4 |
|---|---|
| Application Notes | ELISA: Use at a concentration of 2 µg/ml as the capture reagent in a two-site sandwich ELISA for measuring human IL4 levels. The detection limit is 5 pg/ml ranging up to 3 ng/ml. ICC: Use at an assay dependent dilution. GTX13778 recognizes the secreted (membrane-bound) form of IL4. GTX13778 can be used for intracellular staining of gradient purified human basophils stimulated with IL3. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Purified antibody in phosphate buffered saline, pH 7.4 with 0.1% (w/v) sodium azide. |
