Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody diluted at 1:1500 and competitor's antibody (CST#12153) diluted by 1:3000.Application
IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.Application
Immunofluorescence analysis of methanol-fixed HeLa, using IL6 antibody at 1:200 dilution.| Product Name | IL6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL6 (interleukin-6)This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq] |
| Immunogen | Full length human IL6 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BSF2 antibody HGF antibody HSF antibody IFNB2 antibody IL-6 antibody IL6 antibody interleukin-6 antibody B-cell stimulatory factor 2 antibody interferon beta-2 antibody BSF-2 antibody hybridoma growth factor antibody interleukin BSF-2 antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) |
| Cellular Localization | Secreted |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody diluted at 1:1500 and competitor's antibody (CST#12153) diluted by 1:3000.
Application
IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using IL6 antibody at 1:200 dilution.| Positive Control | recombinant protein IL-6 , IL6-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
