| Product Name | IL7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [7417.111] to IL7Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7417.111 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at a concentration of 1 - 2 μg/ml. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 17 kDa. The exact concentration of antibody required to neutralize recombinant human IL7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity. The detection limit in immunoblotting for recombinant human IL7 is approximately 25 ng/lane and 300 ng/lane under nonreducing and reducing conditions, respectively. A working concentration of the capture antibody at 1-2 μ g/ml (100μl/well) in combination with a biotinylated polyclonal detection antibody at 25-50 ng/ml (100μl/well) is recommended to detect human interleukin 7. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | IL-7 IL 7 |
|---|---|
| Target | IL7 |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at a concentration of 1 - 2 μg/ml. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 17 kDa. The exact concentration of antibody required to neutralize recombinant human IL7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity. The detection limit in immunoblotting for recombinant human IL7 is approximately 25 ng/lane and 300 ng/lane under nonreducing and reducing conditions, respectively. A working concentration of the capture antibody at 1-2 μ g/ml (100μl/well) in combination with a biotinylated polyclonal detection antibody at 25-50 ng/ml (100μl/well) is recommended to detect human interleukin 7. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: PBS |
