Application
Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.Application
Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with IL7R antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | IL7 Receptor alpha (phospho Tyr449) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IL7R (phospho Tyr449)The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). [provided by RefSeq] |
| Immunogen | Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein |
| Modification | p-Tyr449 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB (1:1000 - 1:10000), ELISA (1:100000), IHC-P (5 µg/ml): |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IL7R (phospho Y449) IL7R (phospho Tyr449) IL7R (pY449) IL7R (pTyr449) IL7R phospho Y449 IL7R phospho Tyr449 Phospho IL7R (Y449) Phospho IL7R (Tyr449) Phospho IL7R (pY449) Phospho IL7R (pTyr449) |
|---|---|
| Target | IL7R |
| Tissue Specificity | A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined |
Application
Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.
Application
Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with IL7R antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1:1000 - 1:10000), ELISA (1:100000), IHC-P (5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
