Application
Immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.Application
Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody . Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.| Product Name | IL7 Receptor alpha (phospho Tyr449) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL-7 Receptor alpha Chain (phospho Tyr449)Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein. |
| Modification | p-Tyr449 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD127 IL7RALPHA IL7R |
|---|---|
| Target | IL-7 Receptor Alpha Chain (Phospho Tyr449) |
Application
Immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
Application
Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody . Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
