Application
'Dot Blot of Rabbit anti-IL-7 Receptor antibody. Antigen: IL-7 Receptor and IL-7 Receptor pY449 forms of the immunizing peptide and THRB1 and THRB1 pY171 as controls. Load: 3.0, 0.3, 0.1, 0.05 ug as indicated. Primary antibody: IL-7 Receptor antibody at 1:400 for 45 min at 4°C. Secondary antibody: Dylight488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.'Application
Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with IL7R antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | IL7 Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IL7RInterleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq] |
| Immunogen | Synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 protein |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAIHC-PWB |
| WB (1:1000 - 1:10000), ELISA (1:100000), IHC-P (5 µg/ml): |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD127 IL-7Ralpha MGC107557 IL 7Ralpha IL7Ralpha |
|---|---|
| Tissue Specificity | A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined |
Application
'Dot Blot of Rabbit anti-IL-7 Receptor antibody. Antigen: IL-7 Receptor and IL-7 Receptor pY449 forms of the immunizing peptide and THRB1 and THRB1 pY171 as controls. Load: 3.0, 0.3, 0.1, 0.05 ug as indicated. Primary antibody: IL-7 Receptor antibody at 1:400 for 45 min at 4°C. Secondary antibody: Dylight488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.'
Application
Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with IL7R antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1:1000 - 1:10000), ELISA (1:100000), IHC-P (5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
