Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IMP-3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: PC-3 B: SK-N-SH 7.5% SDS PAGE diluted at 1:1000Application
IMP3 antibody detects IMP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IMP3 protein stained by IMP3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | IMP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IMP-3 (insulin-like growth factor 2 mRNA binding protein 3)The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IMP3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Chicken, BovineMouse (98%), Zebrafish (82%), Xenopus laevis (86%), Chicken (84%), Bovine (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CT98 antibody DKFZp686F1078 antibody IMP-3 antibody IMP3 antibody KOC1 antibody VICKZ3 antibody IGF2BP3 antibody KH domain-containing protein overexpressed in cancer antibody KH domain containing protein overexpressed in cancer antibody IGF II mRN |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IMP-3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: PC-3 B: SK-N-SH 7.5% SDS PAGE diluted at 1:1000
Application
IMP3 antibody detects IMP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IMP3 protein stained by IMP3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | PC-3 , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
