Application
Immunofluorescence analysis of methanol-fixed HeLa, using IMPA1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: JurKat B: Raji 12% SDS PAGE diluted at 1:1000| Product Name | IMPA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IMPA1 (inositol(myo)-1(or 4)-monophosphatase 1)This gene encodes an enyzme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IMPA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (89%), Rat (86%), Pig (81%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IMP antibody IMPA antibody IMPA1 antibody IMPase 1 antibody IMP 1 antibody inositol-1(or 4)-monophosphatase 1 antibody inositol monophosphatase 1 antibody lithium-sensitive myo-inositol monophosphatase A1 antibody inositol(myo)-1(or 4)-monophosphat |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using IMPA1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: JurKat B: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
