Application
IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody . The precipitated IPO13 was detected by diluted at 1:1000.Application
Western Blot analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate. B: IPO13 transfected lysate. 7.5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using Importin 13 antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Importin 13 antibody at 1:200 dilution.Application
Importin 13 antibody [C3], C-term detects Importin 13 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5 % SDS-PAGE Importin 13 antibody [C3], C-term dilution: 1:1000| Product Name | Importin 13 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Importin 13 (importin 13)This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 13. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (100%), Rat (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IMP13 antibody KAP13 antibody KIAA0724 antibody LGL2 antibody RANBP13 antibody IPO13 antibody ran-binding protein 13 antibody karyopherin 13 antibody importin-13 antibody karyopherin-13 antibody late gestation lung 2 antibody Ran binding protein |
|---|---|
| Molecular Weight(MW) | 108 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody . The precipitated IPO13 was detected by diluted at 1:1000.
Application
Western Blot analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate. B: IPO13 transfected lysate. 7.5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using Importin 13 antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Importin 13 antibody at 1:200 dilution.
Application
Importin 13 antibody [C3], C-term detects Importin 13 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5 % SDS-PAGE Importin 13 antibody [C3], C-term dilution: 1:1000| Positive Control | HeLa , Importin 13 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
