Application
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Importin 7 antibody [C3], C-term diluted at 1:500.Application
Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IPO7 protein stained by Importin 7 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000Application
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Importin 7 protein stained by Importin 7 antibody [C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | Importin 7 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Importin 7 (importin 7)The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (100%), Rat (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ14581 antibody Imp7 antibody MGC138673 antibody RANBP7 antibody IPO7 antibody importin-7 antibody RAN-binding protein 7 antibody importin 7 antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Importin 7 antibody [C3], C-term diluted at 1:500.
Application
Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IPO7 protein stained by Importin 7 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000
Application
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Importin 7 protein stained by Importin 7 antibody [C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | A549 , HeLa , Jurkat , NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
