Rabbit polyclonal antibody to INCA1INCA1 was isolated through a yeast triple hybrid approach to identify interaction partners of the cyclin A1-CDK2 heterodimer. INCA1 is evolutionarily conserved and has no homology to any known proteins. Like Cyclin A1, INCA1 is highly expressed in testis with highest levels observed at various points of testis maturation. It has been suggested that the complex of INCA1 and the cyclinA1-CDK2 dimer plays a role in several signaling pathways important for cell cycle control and meiosis. Recent experiments have shown that INCA1 also interacts with the testis protein RSB-66 and this interaction takes place in the cytoplasm.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAICC/IFWB
ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
INCA1 Inhibitor of CDK1 interacting with cyclin A1 HSD45
Target
INCA1
Tissue Specificity
This antibody is specific for INCA1
Application
Application
Western blot analysis of INCA1 in EL4 cell lysate with INCA1 antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunofluorescence of INCA1 in human testis tissue with INCA1 antibody at 20 μg/mL.
Positive Control
Blocking Peptide:
Application Notes
ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.