| Product Name | INCENP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to INCENPChromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex highly basic multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis. INCENP, in complex with the passenger proteins aurora B kinase and survivin, acts at multiple points during mitosis. Studies in knockout mice established INCENP as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis. Targeting of survivin to its centromeric and central spindle locations during mitosis is dependent on INCENP. Direct binding of INCENP to beta tubulin has been demonstrated. Interactions of INCENP with heterochromatin protein HP1Hsa alpha and histone H2A.Z were also reported. INCENP levels are increased in several human cancer lines. |
| Immunogen | Synthetic peptide: SKPRYHKRTSSAVWNSP (with N-terminal added cysteine) conjugated to KLH, corresponding to amino acids 884-901 of Human INCENP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a minimum dilution of 1/2000 (recommended when using a whole extract of mouse NIH-3T3 and rat PC12 cells and a chemiluminescent detection reagent)- 1/5000 (recommended when using a nuclear extract of human HeLa cells and a chemiluminescent detection reagent). Predicted molecular weight: 106 kDa. Detection of the INCENP band by immunoblotting is specifically inhibited with the immunizing peptide. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells. However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Gene Synonyms | FLJ31633 MGC111393 |
|---|---|
| Target | INCENP |
| Cellular Localization | Nuclear |
| Positive Control | Nuclear/whole extract of human HeLa cells |
|---|---|
| Application Notes | WB: Use at a minimum dilution of 1/2000 (recommended when using a whole extract of mouse NIH-3T3 and rat PC12 cells and a chemiluminescent detection reagent)- 1/5000 (recommended when using a nuclear extract of human HeLa cells and a chemiluminescent detection reagent). Predicted molecular weight: 106 kDa. Detection of the INCENP band by immunoblotting is specifically inhibited with the immunizing peptide. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells. However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
