Application
Influenza A Virus H1N1 Hemagglutinin (HA) antibody detects HA (H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: HA (H1N1) protein stained by Influenza A Virus H1N1 Hemagglutinin (HA) antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.Application
Sample (5 µg of whole cell lysate) A: DF1 B: WSN virus infected DF1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Influenza A virus H1N1 HA (Hemagglutinin) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Influenza A Virus H1N1 Hemagglutinin (HA) (Influenza A H1N1 Hemagglutinin(HA)) |
| Immunogen | Recombinant fragment corresponding to C-terminal region of Influenza A virus Hemagglutinin (HA) (A/WSN/1933(H1N1)). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Influenza A |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 64 kDa (note) |
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Application
Influenza A Virus H1N1 Hemagglutinin (HA) antibody detects HA (H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: HA (H1N1) protein stained by Influenza A Virus H1N1 Hemagglutinin (HA) antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
Application
Sample (5 µg of whole cell lysate) A: DF1 B: WSN virus infected DF1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | WSN virus infected DF1 , *influenza virus (A/WSN/33) infected MDCK |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
