Application
Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: M2 protein stained by Influenza A Virus M2 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.Application
Sample (50 µg of whole cell lysate) A: mock DF-1 cell lysates B: WSN-infected DF-1 cell lysates 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Influenza A virus M2 (matrix protein) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Influenza A Virus M2 |
| Immunogen | Recombinant fragment corresponding to Internal region of Influenza A virus M2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Influenza A |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 11 kDa (note) |
|---|
Application
Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: M2 protein stained by Influenza A Virus M2 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
Application
Sample (50 µg of whole cell lysate) A: mock DF-1 cell lysates B: WSN-infected DF-1 cell lysates 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
