Application
Non-infected (–) and infected (+) DF1 whole cell extracts (5 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Influenza A Virus NP antibody detects NP (H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: NP (H1N1) protein stained by Influenza A Virus NP antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.| Product Name | Influenza A virus NP (nucleoprotein) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Influenza A Virus NP |
| Immunogen | Recombinant protein fragment of Influenza A Virus NP (A/WSN/1933(H1N1)) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Influenza A |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Application
Non-infected (–) and infected (+) DF1 whole cell extracts (5 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Influenza A Virus NP antibody detects NP (H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: NP (H1N1) protein stained by Influenza A Virus NP antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.| Positive Control | DF1 (Influenza A virus treatment for 10hr) , *influenza virus (A/WSN/33) infected MDCK |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
