Application
Sample (50 µg of whole cell lysate) A: H1299 B: H1299 infected with influenza virus (MOI=2 h.p.i=8) diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (60 µg of whole cell lysate) A: DF-1(WSN infected cell lysates) B: DF-1 cell lysates diluted at 1:250 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Influenza A Virus PA antibody detects polymerase PA (influenza A H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: polymerase PA (influenza A H1N1) protein stained by Influenza A Virus PA antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.| Product Name | Influenza A virus PA protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Influenza A Virus PA (Influenza A virus ( H1N1) polymerase PA) |
| Immunogen | Recombinant fragment corresponding to C-terminal region of Influenza A virus PA (A/WSN/1933(H1N1)). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Influenza A |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 83 kDa (note) |
|---|
Application
Sample (50 µg of whole cell lysate) A: H1299 B: H1299 infected with influenza virus (MOI=2 h.p.i=8) diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (60 µg of whole cell lysate) A: DF-1(WSN infected cell lysates) B: DF-1 cell lysates diluted at 1:250 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Influenza A Virus PA antibody detects polymerase PA (influenza A H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: polymerase PA (influenza A H1N1) protein stained by Influenza A Virus PA antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.| Positive Control | Influenza virus- infected H1299 lysates , Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates , *influenza virus (A/WSN/33) infected MDCK cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
